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Image Search Results
Journal: Frontiers in Physiology
Article Title: Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals
doi: 10.3389/fphys.2021.773995
Figure Lengend Snippet: Alterations in select mitochondrial enzymes with CON vs. VAR training. Data presented in arbitrary units (ADUs) include isocitrate dehydrogenase 2 (IDH2) protein levels from the sarcoplasmic protein pool (panel A ), carnitine palmitoyltransferase 1 (CPT1) protein levels from the sarcoplasmic protein pool (panel B ), and very long-chain specific acyl-CoA dehydrogenase (VLCAD) protein levels from the sarcoplasmic protein pool (panel C ). Data are presented as means with standard deviation bars. Panel (D) contains representative Western blot images for each target. Note that two of Ponceau images in panel (D) (for IDH2 and VLCAD) are the same given that the same subject was used for the representative Western blot images of these targets.
Article Snippet: Membranes were then incubated overnight at 4°C with the following antibody cocktails (each at a 1:1,000 dilution) in TBST with 5% bovine serum albumin (BSA): (i) goat anti-human creatine kinase (CKM; Abcam; Cambridge, MA, United States; cat# ab174672), (ii) rabbit anti-human L-type amino acid transporter (LAT1, Cell Signaling Technology; Danvers, MA, United States; cat# 5347), (iii) rabbit anti-human glucose transporter 4 (GLUT4; Cell Signaling Technology, cat# 2213), (iv) rabbit anti-human lactate dehydrogenase (LDHA; Cell Signaling Technology, cat# 2012), (v) rabbit anti-human phosphofructokinase (PFKM; Abcam, cat#: ab154804), (vi) rabbit anti-human total oxidative phosphorylation (OXPHOS) cocktail (Abcam, cat# ab110411), (vii) rabbit anti-human complex IV (Cell Signaling Technology, cat# 4850), (
Techniques: Standard Deviation, Western Blot